推拿疗法在早期干预运动发育迟缓患儿中的应用价值

目的 研究推拿疗法在早期干预运动发育迟缓患儿中的应用价值,为临床治疗提供参考依据。方法 选取2015年7月-2016年6月期间在寿光市妇幼保健院就诊的运动发育迟缓患儿为研究对象,并根据随机数字表法将其分为对照组和干预组,对照组予常规治疗,干预组在此基础上给予中医推拿进行干预。观察两组患儿的干预效果,比较两组患儿干预前后Gesell运动能评分和发育商的差异。结果 干预组患儿治疗有效率为93.3%,高于对照组;干预后,干预组Gesell运动能评分高于对照组(t=-12.524,P<0.001),干预组患儿发育商高于对照组,但IL-6、hs-CRP和TNF-α水平低于对照组(P<0.05)。结论 推拿疗法早期干预对运动发育迟缓的患儿临床疗效明显,且干预越早效果越好,具有良好的应用价值。     

乳腺疏导推拿配合中药外敷治疗哺乳期急性乳腺炎的疗效观察

目的观察运用乳腺疏导推拿配合中药外敷治疗哺乳期急性乳腺炎患者的疗效。方法将符合标准的60例哺乳期急性乳腺炎患者,随机分成3组,推拿组、外敷组及观察组各20例。推拿组给予乳腺疏导推拿治疗,外敷组给予中药外敷治疗(乳香、香附、郁金、生白芷、青皮及皂角刺等),观察组给予乳腺疏导推拿配合中药外敷治疗。观察3组治疗1个疗程后的有效率、临床症状的恢复时间、白细胞(WBC)及C-反应蛋白(CRP)水平。结果 3组治疗前后的有效率、临床症状的恢复时间、WBC、CRP比较,差异均有统计学意义(均P<0.05),观察组明显优于推拿组及外敷组(P<0.05)。结论乳腺疏导推拿和中药外敷都可以改善哺乳期急性乳腺炎患者临床症状、WBC及CRP水平,但乳腺疏导推拿配合中药外敷的疗效更加显著。     

The intraocular staining potential of anthocyanins and their retinal biocompatibility: a preclinical study

Purpose: To perform preclinical studies to determine the efficacy and safety of anthocyanins as stains for the internal limiting membrane (ILM) of the eye.

Materials and methods: Cyanidin (Cya), delphinidin (Del), luteolinidin (Lut), peonidin (Peo) and pelargonidin (Pel) were evaluated. These natural dyes were used to stain the lens capsule and ILM of pig eyes. The effects of these dyes on retinal cell viability was determined using a water-soluble tetrazolium salt assay, and oxidative stress was measured in vitro. Histopathology, in situ TUNEL labelling, transmission electronic microscopy (TEM), and electroretinography (ERG) were performed on rats following the intravitreal and subretinal injection of the neuroprotective dyes.

Results: All anthocyanins stained the lens capsule and ILM of the pigs at a concentration of 1?mg/ml. Del, Lut and Peo were non-toxic and produced survival rates in the ARPE19 and RGC5 cells that were similar to those in control cells. We treated eyes with H₂O₂ and three dyes (Del, Lut, and Peo) to explore the possible neuroprotective effects and observed significantly higher survival rates in the ARPE19 cells treated with Del, Lut or Peo and the RGC5 cells treated with Lut or Peo than those in the control cells. Three dyes were intravitreally and subretinally injected into rats in vivo, and the histology showed mildly disorganized retinal cell layers. TUNEL staining and TEM examinations did not reveal additional toxic effects. Rat ERGs were not altered after intravitreal injections.

Conclusions: This preclinical study, Del, Lut, and Peo show potential as staining agents and warrant further investigation as vital dyes.     

Are rats more human than mice?

In contrast to rats, mouse models are nowadays generally used for the investigation of immune responses and immune-mediated diseases, there are many different strains and mouse-specific tools available, and it is easy to generate transgenic and constitutive or inducible knockout mice for any gene. Many immune markers and mechanisms have been detected in mice and have been introduced as gold standard in immunology, however, some turned out to be not unconditionally transferable to the human immune system.Rats have been used more frequently in former days but are mostly outstripped by mice due to the fact that fewer strains are available, they need more space than mice, are more expensive to maintain and breed, and it is extremely difficult to generate transgenic or ko-rats. Consequently, the choice of rat-specific diagnostic tools like antibodies is quite poor and most researchers have switched to mouse models for the investigation of immune mechanisms, while rats are still widely used for toxicology by the pharmaceutical industry. However, it should be taken into consideration that there are some immunological similarities between rats and humans that are not presented in mice. Some of them like MHC class II and Foxp3 expression by activated effector T cells we have detected during our research on the immune response of rat models of experimental autoimmune uveitis.     

Functional characterization of CEP250 variant identified in nonsyndromic retinitis pigmentosa

Retinitis pigmentosa (RP) is the most common manifestation of inherited retinal diseases with high degree of genetic, allelic, and phenotypic heterogeneity. CEP250 encodes the C‐Nap1 protein and has been associated with various retinal phenotypes. Here, we report the identification of a mutation (c.562C>T, p.R188*) in the CEP250 in a consanguineous family with nonsyndromic RP. To gain insights into the molecular pathomechanism underlying CEP250 defects and the functional relevance of CEP250 variants in humans, we conducted a functional characterization of CEP250 variant using a novel Cep250 knockin mouse line. Remarkably, the disruption of Cep250 resulted in severe impairment of retinal function and significant retinal morphological alterations. The homozygous knockin mice showed significantly reduced retinal thickness and ERG responses. This study not only broadens the spectrum of phenotypes associated with CEP250 mutations, but also, for the first time, elucidates the function of CEP250 in photoreceptors using a newly established animal model.     

视网膜色素上皮氧化损伤SIRT1表达改变

目的明确氧化应激对视网膜色素上皮细胞(RPE)中去乙酰化酶1(SIRT1)表达的影响。方法以人RPE细胞为实验对象,不同浓度H_2O_2(0、200、300μmol/L)处理RPE细胞,观察处理后24 h细胞形态的改变情况,检测处理后24 h与72 h细胞中SIRT1的mRNA与蛋白表达情况。结果 H_2O_2作用后,随H_2O_2浓度的增加,RPE细胞的形态受损,有凋亡小体的出现;在氧化应激24 h后细胞内SIRT1的转录水平增加,而在氧化应激72 h后SIRT1的蛋白表达显著下降。结论氧化应激可导致RPE细胞形态改变,SIRT1在RPE细胞内维持着氧化与抗氧化应激系统平衡,因此将SIRT1可作为临床上年龄相关性黄斑变性病(AMD)治疗的靶点。     

毫针浅刺法配合推拿治疗原发性失眠临床研究

目的:观察毫针浅刺法配合推拿治疗原发性失眠的临床疗效。方法:选取2015年1月—2015年12月在本院就诊的原发性失眠患者90例,按照随机数字表法分为对照组和治疗组,每组45例。对照组给予普通针刺结合推拿治疗,治疗组给予毫针浅刺法配合推拿治疗。结果:治疗组治疗后愈显率62.22%,治疗后1个月愈显率71.11%,对照组治疗后愈显率40.00%,治疗后1个月愈显率35.56%,治疗组优于对照组(P0.05);治疗组治疗后、治疗后1个月PSQI评分均优于对照组(P0.05)。结论:毫针浅刺法配合推拿治疗原发性失眠疗效显著。     

青光眼视神经节细胞内外源性凋亡机制及中西医保护研究新进展

从青光眼视神经细胞(RGC)凋亡的内源性、外源性凋亡途径的部分机制,现代西医、中医药保护青光眼RGC凋亡的临床及基础研究报道入手探讨青光眼RGC的防治手段,指出当今关于中医药防治青光眼视功能损伤的临床及基础研究日益增多,但针对青光眼RGC凋亡和改善的中医药相关文献报道仍然较少,其作用机理也有待进一步证实。     

中医康抚护理对腹腔镜术后腹胀及疼痛的影响

目的:观察中医康抚护理对腹腔镜术后患者腹胀及疼痛的影响。方法:将80例患者随机分为2组,每组40例,对照组实施常规护理,观察组在此基础上给予中医康抚护理,比较2组干预过程中VAS评分及肛门排气、排便时间和肠鸣音恢复时间、腹胀缓解时间。结果:VAS评分干预24小时后2组即开始降低,与干预前比较差异有统计学意义(P0.05);干预后相同时间2组比较,差异也有统计学意义(P0.05)。肠鸣音恢复时间、肛门排气时间、经肛门排便时间及腹胀缓解时间2组比较,差异有统计学意义(P0.05)。腹胀缓解率观察组为90.0%,对照组为67.5%,2组比较差异均有统计学意义(P0.05)。结论:中医康抚护理能有效的缓解腹腔镜术后患者腹胀不适,促进术后胃肠功能早期恢复,加速经肛门排气排便时间。